Web12 apr. 2024 · For all cell lines, culture media were changed every 48 to 72 h after washing with 1% PBS. Cells were split by adding 1 mL trypsin once they reached 60 to 70% confluency. Cells were maintained at 37 °C and 5% CO2. The hydrocortisone, insulin, and EGF were purchased from Sigma-Aldrich (Sigma-Aldrich Canada Co., Oakville, ON, …
Hydrocortisone 21-hemisuccinate powder, BioReagent, cell culture ...
WebHydrocortisone Stock Solution is recommended for use as a cell culture supplement and for the preparation of PneumaCult™, MammoCult™, and EpiCult™ media when culturing airway epithelial cells, mammary primary cells, or tumor cell lines. Hydrocortisone … MammoCult™ Medium (Human) is a serum-free culture medium optimized for … EpiCult™-B Human Medium Kit is a serum-free culture medium optimized for the … This produces a highly pure sample ready for cell culture and mammary colony … PneumaCult™-Ex is a defined, serum- and BPE-free cell culture medium that … See More Culture primary airway epithelial cells in an improved serum- and bovine … See More Model the airway epithelium using air-liquid interface (ALI) cultures to … See More Identify and characterize mammary epithelial stem, progenitor … Tissue culture-treated cell culture flask; T-75, T-150 and T-175 cm² formats Quick … WebHydrocortisone is used for the following applications: Used in myogenic induction and identification; Used in long-term stromal cultures and methylcellulose cultures (one of … black clutches for wedding
Hydrocortisone Stock Solution (96 µg/mL) - STEMCELL
WebIn our study, we evaluated the influence of epidermal growth factor (EGF) and hydrocortisone (HC), often supplemented in endothelial cell (EC) specific media, on the … Web15 apr. 2024 · The mitochondrial evaluation by MTT aimed to measure possible influences of incubation with hydrocortisone, in concentrations of 0.5, 1.0, 1.5, 2.0, and 2.5 µM, in HEp-2 cell culture, relating possible findings to the population density results which were then analyzed. Figure 1 presents the results of the mitochondrial activity. Web18 mei 2015 · In this research, a sensitive and reliable LC-MS/MS method was developed and applied to determine the concentration of triptolide in rat plasma, microsomes, and cell incubation media. The absolute oral bioavailability of triptolide is 63.9% at a dose of 1 mg·kg−1. In vitro, the bidirectional transport of triptolide across Caco-2 cells was … galvanised mesh fencing